Odrun Arna Gederaas

• Gederaas, Odrun Arna; Lindgren, Mikael; Stigen, Kari; Høgset, Anders Skog & Hirschberg, Henry (2024). Pharmacokinetics of disulphonated tetraphenyl chlorin (fimaporfin/TPCS<inf>2a</inf>) in a rat glioma model - methodology. Journal of Porphyrins and Phthalocyanines. ISSN 1088-4246. doi: 10.1142/S1088424624500238.
• Gederaas, Odrun Arna; Sharma, Animesh; Mbarak, Saide; Sporsheim, Bjørnar; Høgset, Anders & Bogoeva, Vanya [Vis alle 8 forfattere av denne artikkelen] (2023). Proteomic analysis reveals mechanisms underlying increased efficacy of bleomycin by photochemical internalization in bladder cancer cells. Molecular Omics. ISSN 2515-4184. 19(7), s. 585–597. doi: 10.1039/d2mo00337f. Fulltekst i vitenarkiv Vis sammendrag

  Photochemical internalization (PCI) is a promising new technology for site-specific drug delivery, developed from photodynamic therapy (PDT). In PCI, light-induced activation of a photosensitizer trapped inside endosomes together with e.g. chemotherapeutics, nucleic acids or immunotoxins, allows cytosolic delivery and enhanced local therapeutic effect. Here we have evaluated the photosensitizer meso-tetraphenyl chlorine disulphonate (TPCS2a/fimaporfin) in a proteome analysis of AY-27 rat bladder cancer cells in combination with the chemotherapeutic drug bleomycin (BML). We find that BLMPCI attenuates oxidative stress responses induced by BLM alone, while concomitantly increasing transcriptional repression and DNA damage responses. BLMPCI also mediates downregulation of bleomycin hydrolase (Blmh), which is responsible for cellular degradation of BLM, as well as several factors known to be involved in fibrotic responses. PCI-mediated delivery might thus allow reduced dosage of BLM and alleviate unwanted side effects from treatment, including pulmonary fibrosis.

• Hageberg, Ingvild Uri; Arja, Katriann; Vindstad, Benedikte Emilie; Bergvoll, Johannes; Gederaas, Odrun Arna & Melø, Thor Bernt [Vis alle 8 forfattere av denne artikkelen] (2023). Photophysics of Glycosylated Ring-Fused Chlorin Complexes and Their Photosensitizing Effects on Cancer Cells. ChemPhotoChem. ISSN 2367-0932. 7(8). doi: 10.1002/cptc.202300028. Vis sammendrag

  The future of photodynamic therapy (PDT) as a promising cancer treatment relies on the development of new selective and effective photosensitizers (PS) with improved photophysical and biochemical qualities. Herein, we present the synthetic procedure, photophysical properties and photosensitizing effects of novel glycosylated 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridine] fused chlorin agents featuring either glucose, galactose, or N-acetyl glucosamine. It is shown that both the proto- and Zn2+-ion forms of the ring-stabilized glycochlorins exhibit the required photophysical properties in terms of triplet excited states and singlet oxygen generation, the latter more than 50 % in organic solvents such as CHCl3. Employing the rat AY-27 and human T24 cancer cell models, it was found that these are superior to the corresponding unglycosylated chlorin in biological activity, and moreover, the proto form is 2–3 times superior to the Zn-stabilized variant. Provisional flow cytometry and cell localization studies of the proto-form indicate both necrotic and apoptotic biological activity, and that the photosensitizer localizes in the mitochondria, cell membrane and lysosomes. However, the localization into the lysosomes is dominating and increases substantially with time. We anticipate the findings will aid in the development of photosensitizers for targeted cancer PDT.

• Desgranges, Stéphane; Juzenas, Petras; Vasovic, Vlada; Gederaas, Odrun Arna; Lindgren, Mikael & Warloe, Trond [Vis alle 8 forfattere av denne artikkelen] (2022). Amphiphilic Protoporphyrin IX Derivatives as New Photosensitizing Agents for the Improvement of Photodynamic Therapy. Biomedicines. 10(2). doi: 10.3390/biomedicines10020423. Fulltekst i vitenarkiv
• Gederaas, Odrun Arna; Sørensen, Andreas; Lindgren, Mikael; Melø, Thor Bernt; Altin, Dag & Flatby, Ellen M. Samdahl [Vis alle 8 forfattere av denne artikkelen] (2022). Synthesis and in vitro evaluation of a novel thienopyrimidine with phototoxicity towards rat glioma F98 cells. Journal of Photochemistry and Photobiology. ISSN 2666-4690. 10. Fulltekst i vitenarkiv
• Nguyen, Jimmi; Madsen J, Steen; Gederaas, Odrun Arna; Berg, Kristian; Høgset, Anders & Hirschberg, Henry (2020). Ultrasonic Activation of the Sonosensitizer Fimaporfin Enhances the Efficacy of Chemotherapy: An In Vitro Study on Rat Glioma Cells. . Journal of Cancer Research and Clinical Oncology. ISSN 0171-5216. doi: 10.31487/j.COR.2020.05.10.
• Callaghan, Susan; Vindstad, Benedikte Emilie; Flanagan, Keith J.; Melø, Thor Bernt; Lindgren, Mikael & Grendstad, Kristin [Vis alle 8 forfattere av denne artikkelen] (2020). Structural, photophysical, and photobiological studies on BODIPY‐anthracene dyads. ChemPhotoChem. ISSN 2367-0932. doi: 10.1002/cptc.202000191.
• Shi, L.; Pohla, H.; Buchner, A.; Zhang, L.; Pongratz, T. & Ruhm, A. [Vis alle 11 forfattere av denne artikkelen] (2020). MOP-dependent enhancement of methadone on the effectiveness of ALA-PDT for A172 cells by upregulating phosphorylated JNK and BCL2. Photodiagnosis and Photodynamic Therapy. ISSN 1572-1000. 30. doi: 10.1016/j.pdpdt.2020.101657.
• Einrem, Rune F.; Alemayehu, Abraham; Borisov, Sergey M.; Ghosh, Abhik & Gederaas, Odrun Arna (2020). Amphiphilic rhenium-oxo corroles as a new class of sensitizers for photodynamic therapy. ACS Omega. ISSN 2470-1343. 5(18), s. 10596–10601. doi: 10.1021/acsomega.0c01090. Fulltekst i vitenarkiv
• Lindgren, Mikael; Gederaas, Odrun Arna; Siksjø, Monica; Hansen, Tom Andre; Chen, Lena & Mettra, Bastien [Vis alle 8 forfattere av denne artikkelen] (2020). Influence of Polymer Charge on the Localization and Dark- and Photo-Induced Toxicity of a Potential Type I Photosensitizer in Cancer Cell Models. Molecules. ISSN 1431-5157. 25(5). doi: 10.3390/molecules25051127. Fulltekst i vitenarkiv
• Gederaas, Odrun Arna; Husebye, Harald; Johnsson, Anders Carl G.; Callaghan, Susan & Brunsvik, Anders (2019). In vitro and in vivo effects of HAL on porphyrin production in rat bladder cancer cells (AY27) . Journal of Porphyrins and Phthalocyanines. ISSN 1088-4246. 23, s. 813–820. doi: 10.1142/S1088424619500615.
• Shi, Lei; Buchner, Alexander; Pohla, Heike; Pongratz, Thomas; Rühm, Adrian & Zimmermann, Wolfgang [Vis alle 11 forfattere av denne artikkelen] (2019). Methadone enhances the effectiveness of 5‐aminolevulinic acid‐based photodynamic therapy for squamous cell carcinoma and glioblastoma in vitro. Journal of Biophotonics. ISSN 1864-063X. 12:201800468(10), s. 1–9. doi: 10.1002/jbio.201800468.
• Søgaard, Caroline Krogh; Blindheim, Augun; Røst, Lisa Marie; Petrovic, Voin; Nepal, Anala & Bachke, Siri [Vis alle 12 forfattere av denne artikkelen] (2018). "Two hits - one stone" increased efficacy of cisplatin-based therapies by targeting PCNA's role in both DNA repair and cellular signaling. OncoTarget. ISSN 1949-2553. 9(65), s. 32448–32465. doi: 10.18632/oncotarget.25963. Fulltekst i vitenarkiv
• Holien, Toril; Gederaas, Odrun Arna; Darvekar, Sagar; Christensen, Eidi & Qian, Peng (2018). Comparison Between 8-Methoxypsoralen and 5-Aminolevulinic Acid in Killing T Cells of Photopheresis Patients Ex Vivo. Lasers in Surgery and Medicine. ISSN 0196-8092. 50(5), s. 469–475. doi: 10.1002/lsm.22806. Fulltekst i vitenarkiv
• Haug, Markus; Brede, Gaute; Håkerud, Monika; Nedberg, Anne Grete Gargul; Gederaas, Odrun Arna & Flo, Trude Helen [Vis alle 10 forfattere av denne artikkelen] (2018). Photochemical internalization of peptide antigens provides a novel strategy to realize therapeutic cancer vaccination. Frontiers in Immunology. ISSN 1664-3224. 9:650, s. 1–14. doi: 10.3389/fimmu.2018.00650. Fulltekst i vitenarkiv Vis sammendrag

  Effective priming and activation of tumor-specific CD8+ cytotoxic T lymphocytes (CTLs) is crucial for realizing the potential of therapeutic cancer vaccination. This requires cytosolic antigens that feed into the MHC class I presentation pathway, which is not efficiently achieved with most current vaccination technologies. Photochemical internalization (PCI) provides an emerging technology to route endocytosed material to the cytosol of cells, based on light-induced disruption of endosomal membranes using a photosensitizing compound. Here we investigated the potential of PCI as a novel, minimally invasive and well-tolerated vaccination technology to induce priming of cancer-specific CTL responses to peptide antigens. We show that PCI effectively promotes delivery of peptide antigens to the cytosol of antigen presenting cells (APCs) in vitro. This resulted in a 30-fold increase in MHC class I/peptide complex formation and surface presentation, and a subsequent 30-100-fold more efficient activation of antigen-specific CTLs compared to using the peptide alone. The effect was found to be highly dependent on the dose of the PCI treatment, where optimal doses promoted maturation of immature dendritic cells, thus also providing an adjuvant effect. The effect of PCI was confirmed in vivo by the successful induction of antigen-specific CTL responses to cancer antigens in C57BL/6 mice following intradermal peptide vaccination using PCI technology. We thus show new and strong evidence that PCI technology holds great potential as a novel strategy for improving the outcome of peptide vaccines aimed at triggering cancer-specific CD8+ CTL responses.

• Gederaas, Odrun Arna; Johnsson, Anders Carl G.; Berg, Kristian; Manandhar, Rojlina; Shrestha, Chetana & Skåre, Daniel [Vis alle 9 forfattere av denne artikkelen] (2017). Photochemical internalization in bladder cancer-development of an orthotopic in vivo model. Photochemical and Photobiological Sciences. ISSN 1474-905X. 16(11), s. 1664–1676. doi: 10.1039/c7pp00176b.
• Chatzisideri, T; Thysiadis, S; Katsamakas, S; Dalezis, P; Sigala, I & Lazarides, T [Vis alle 11 forfattere av denne artikkelen] (2017). Synthesis and biological evaluation of a Platinum(II)-c(RGDyK) conjugate for integrin-targeted photodynamic therapy. European Journal of Medicinal Chemistry. ISSN 0223-5234. 141, s. 221–231. doi: 10.1016/j.ejmech.2017.09.058.
• Alemayehu, Abraham; Day, Nicholas U.; Mani, Tomoyasu; Rudine, Alexander B.; Kolle, Ekaney Thomas & Gederaas, Odrun Arna [Vis alle 9 forfattere av denne artikkelen] (2016). Gold Tris(carboxyphenyl)corroles as Multifunctional Materials: Room Temperature Near-IR Phosphorescence and Applications to Photodynamic Therapy and Dye-Sensitized Solar Cells. ACS Applied Materials & Interfaces. ISSN 1944-8244. 8(29), s. 18935–18942. doi: 10.1021/acsami.6b04269.
• Bogoeva, Vanya; Siksjø, Monica; Sæterbø, Kristin Grendstad; Melø, Thor Bernt; Bjørkøy, Astrid & Lindgren, Mikael [Vis alle 7 forfattere av denne artikkelen] (2016). Ruthenium porphyrin-induced photodamage in bladder cancer cells. Photodiagnosis and Photodynamic Therapy. ISSN 1572-1000. 14, s. 9–17. doi: 10.1016/j.pdpdt.2016.01.012.
• Helander, Linda; Sharma, Animesh; Krokan, Hans Einar; Plaetzer, Kristjan; Krammer, Barbara & Tortik, Nicole [Vis alle 9 forfattere av denne artikkelen] (2016). Photodynamic treatment with hexyl-aminolevulinate mediates reversible thiol oxidation in core oxidative stress signaling proteins. Molecular Biosystems. ISSN 1742-206X. doi: 10.1039/c5mb00744e.
• Baglo, Yan; Qian, Peng; Hagen, Lars; Berg, Kristian; Høgset, Anders & Drabløs, Finn [Vis alle 7 forfattere av denne artikkelen] (2015). Studies of the photosensitizer disulfonated meso-tetraphenyl chlorin in an orthotopic rat bladder tumor model. Photodiagnosis and Photodynamic Therapy. ISSN 1572-1000. 12(1), s. 58–66. doi: 10.1016/j.pdpdt.2014.12.005.
• Gederaas, Odrun Arna; Hauge, Anette; Ellingsen, Pål Gunnar; Berg, Kristian; Altin, Dag & Bardal, Tora [Vis alle 8 forfattere av denne artikkelen] (2015). Photochemical internalization of bleomycin and temozolomide – in vitro studies on the glioma cell line F98. Photochemical and Photobiological Sciences. ISSN 1474-905X. 14(7), s. 1357–1366. doi: 10.1039/c5pp00144g.
• Gederaas, Odrun Arna; Søgaard, Caroline Danielsen; Viset, Trond; Bachke, Siri; Bruheim, Per & Arum, Carl-Jørgen [Vis alle 7 forfattere av denne artikkelen] (2014). Increased anticancer efficacy of intravesical mitomycin C therapy when combined with a PCNA targeting peptide. Translational Oncology. ISSN 1944-7124. 7(6), s. 812–823. doi: 10.1016/j.tranon.2014.10.005. Fulltekst i vitenarkiv
• Helander, Linda; Krokan, Hans Einar; Johnsson, Anders Carl G.; Gederaas, Odrun Arna & Plaetzer, Kristjan (2014). Red versus blue light illumination in hexyl 5-aminolevulinate photodynamic therapy: the influence of light color and irradiance on the treatment outcome in vitro. Journal of Biomedical Optics. ISSN 1083-3668. 19:088002(8). doi: 10.1117/1.JBO.19.8.088002. Fulltekst i vitenarkiv Vis sammendrag

  Abstract. Hexyl 5-aminolevulinate (HAL) is a lipophilic derivative of 5-aminolevulinate, a key intermediate in biosynthesis of the photosensitizer protoporphyrin IX (PpIX). The photodynamic efficacy and cell death mode after red versus blue light illumination of HAL-induced PpIX have been examined and compared using five different cancer cell lines. LED arrays emitting at 410 and 624 nm served as homogenous and adjustable light sources. Our results show that the response after HAL-PDT is cell line specific, both regarding the shape of the dose-survival curve, the overall dose required for efficient cell killing, and the relative amount of apoptosis. The ratio between 410 and 624 nm in absorption coefficient correlates well with the difference in cell killing at the same wavelengths. In general, the PDT efficacy was several folds higher for blue light as compared with red light, as expected. However, HAL-PDT624 induced more apoptosis than HAL-PDT410 and illumination with low irradiance resulted in more apoptosis than high irradiance at the same lethal dose. This indicates differences in death modes after low and high irradiance after similar total light doses. From a treatment perspective, these differences may be important.

• Baglo, Yan; Hagen, Lars; Høgset, Anders; Drabløs, Finn; Otterlei, Marit & Gederaas, Odrun Arna (2014). Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization. BioMed Research International. ISSN 2314-6133. 14(74). doi: 10.1155/2014/921296. Fulltekst i vitenarkiv Vis sammendrag

  Bleomycin is a cytotoxic chemotherapeutic agent widely used in cancer treatment. However, its efficacy in different cancers is low, possibly due to limited cellular internalization. In this study, a novel approach known as photochemical internalization (PCI) was explored to enhance bleomycin delivery in bladder cancer cells (human T24 and rat AY-27), as bladder cancer is a potential indication for use of PCI with bleomycin. The PCI technique was mediated by the amphiphilic photosensitizer disulfonated tetraphenyl chlorin (TPCS2a) and blue light (435 nm). Two additional strategies were explored to further enhance the cytotoxicity of bleomycin; a novel peptide drug ATX-101 which is known to impair DNA damage responses, and the protease inhibitor E-64 which may reduce bleomycin degradation by inhibition of bleomycin hydrolase. Our results demonstrate that the PCI technique enhances the bleomycin effect under appropriate conditions, and importantly we show that PCI-bleomycin treatment leads to increased levels of DNA damage supporting that the observed effect is due to increased bleomycin uptake. Impairing the DNA damage responses by ATX-101 further enhances the efficacy of the PCI-bleomycin treatment, while inhibiting the bleomycin hydrolase does not.

• Amundsen, Tore; Sundstrøm, Stein Harald; Buvik, Turid; Gederaas, Odrun & Haaverstad, Rune (2014). Can dogs smell lung cancer? First study using exhaled breath and urine screening in unselected patients with suspected lung cancer. Acta Oncologica. ISSN 0284-186X. 53(3), s. 307–315. doi: 10.3109/0284186X.2013.819996.

Se alle arbeider i Cristin

• Swaminathan, Priyanka; Sundnes, Marikken L; Lindgren, Mikael; Gederaas, Odrun Arna; Klingstedt, Thérese & Nyström, Sofie [Vis alle 9 forfattere av denne artikkelen] (2024). Hyperspectral imaging of amyloid asynuclein(asyn) in HEK293 cells using Luminescent conjugated oligothiophenes(LCOs).
• Swaminathan, Priyanka; Lindgren, Mikael & Gederaas, Odrun Arna (2024). Use of in-vitro cell models and protein fluorescent markers for studying amyloid related diseases.
• Swaminathan, Priyanka; Sundnes, Marikken L; Vindstad, Benedikte; Gederaas, Odrun Arna; Lindgren, Mikael & Klingstedt, Thérese [Vis alle 10 forfattere av denne artikkelen] (2023). Use of in-vitro cell-models in understanding amyloidosis.
• Sundnes, Marikken L; Swaminathan, Priyanka; Gederaas, Odrun Arna; Lindgren, Mikael; Klingstedt, Thérese & Hellstrand, Ebba [Vis alle 10 forfattere av denne artikkelen] (2023). Hyperspectral microscopy and ligand screening of TTR amyloid fibrils.
• Gederaas, Odrun Arna; Høgset, Anders & Lindgren, Olof Mikael (2023). In vivo and in vitro models for photodynamic therapy by using novel photosensitizers. Photodiagnosis and Photodynamic Therapy. ISSN 1572-1000. 41. doi: 10.1016/j.pdpdt.2023.103435.
• Gederaas, Odrun Arna (2021). Photodynamic Therapy (PDT)- in vitro and in vivo studies using novel photosensitizers.
• Gederaas, Odrun Arna; Callaghan, Susan; Vindstad, Benedikte E.; Flanaghan, Keith J.; Melø, Thor Bernt & Sæterbø, Kristin G. [Vis alle 8 forfattere av denne artikkelen] (2020). Photobiological studies on a novel lead BODIPY-anthracene dyad for bladder cancer therapy.
• Gederaas, Odrun Arna; Sørensen, Andreas S.; Lindgren, Mikael & Hoff, Bård Helge (2020). Prosperities of a novel and photosensitive molecule.
• Gederaas, Odrun Arna (2020). Prosperities of a novel photosensitive molecule.
• Gederaas, Odrun Arna & Hagen, Lars (2020). Drug delivery and endosomal drug quantification of Doxorubicin post photochemical internalization.
• Gederaas, Odrun Arna; Johnsson, Anders Carl G. & Lindgren, Mikael (2019). Årsmøte og Faglig møte i NOFFOF (Norsk Forening For Fotobiologi og Fotomedsin). Dato: 1.nov. 2019 Seminar med foredrag- 20 deltagere. Odrun Gederaas var hovedansvarlig for arrangementet.
• Gederaas, Odrun Arna; Granja, Andreia; Hansen, Tom Andre; Lindgren, Mikael; Pinheiro, M. & Sousa, C. T. [Vis alle 7 forfattere av denne artikkelen] (2019). Assessing the influence of gold nanoparticles in photodynamic therapy using singlet oxygen sensitizers .
• Gederaas, Odrun Arna; Saidshahagha, Orjahan; Høgset, Anders & Hagen, Lars (2019). Quantification of Doxorubicin in endosomes of rat bladder cancer cells (AY27) post photochemical internalization.
• Gederaas, Odrun Arna; Johnsson, Anders Carl G.; Høgset, Anders; Hjelde, Astrid & Lindgren, Mikael (2019). Novel Technologies by Photodynamic Therapy (PDT).
• Gederaas, Odrun Arna (2019). Fotodynamisk radioterapi for blærekreft.
• Gederaas, Odrun Arna; Johnsson, Anders; Berg, Kristian; Manandhar, Rojlina; Shrestha, Chetana & Skåre, Daniel [Vis alle 9 forfattere av denne artikkelen] (2019). Effects of Photochemical Internalization (PCI) - Bladder Cancer, in vitro and in vivo.
• Gederaas, Odrun Arna; Johnsson, Anders Carl G.; Berg, Kristian; Manandhar, Rojlina; Shrestha, Chetana & Skåre, Daniel [Vis alle 9 forfattere av denne artikkelen] (2019). Photochemical Internalization (PCI) - Bladder Cancer.
• Gederaas, Odrun Arna (2019). Interdisiplinary research including pharmacy.
• Gederaas, Odrun Arna (2018). Interdisiplinary research including pharmacy.
• Gederaas, Odrun Arna (2018). Psoralens and porphyrin precursors in medical treatment.
• Gederaas, Odrun Arna; Holien, Toril; Darvekar, Sagar; Christensen, Eidi & Qian, Peng (2018). Comparison between 8-methoxypsoralen and 5-aminolevulinic acid in killing T cells of photopheresis patients ex vivo.
• Gederaas, Odrun Arna; Einrem, Rune Finsås; Alemayehu, Abraham; Bogoeva, Vanya; Ghosh, Abhik & Lindgren, Mikael (2018). Photodynamic therapy using corroles and ruthenium porphyrins.
• Haug, Markus; Brede, Gaute; Håkerud, Monika; Nedberg, A; Gederaas, Odrun Arna & Edwards, Victoria Tudor [Vis alle 10 forfattere av denne artikkelen] (2018). Photochemical internalization as a novel strategy to realize therapeutic cancer peptide vaccination.
• Singh, Rinku; Hals, Ingrid Kathrin; Høgset, Anders; Grill, Valdemar Erik Robert & Gederaas, Odrun Arna (2018). Increased toxicity of amylin (Islet Amyloid Polypeptide) in beta cells induced by photochemical internalization. Photodiagnosis and Photodynamic Therapy. ISSN 1572-1000. 23, s. 218–220. doi: 10.1016/j.pdpdt.2018.06.017.
• Gederaas, Odrun Arna (2018). Photodynamic therapy (PDT) - in vitro and in vivo experiments.
• Gederaas, Odrun Arna; Siksjø, Monika & Kanstad, John Gederaas (2018). "Cell culturing using three different light sources" Video: Link: https://youtu.be/RY9wWccZXGk.
• Gederaas, Odrun Arna; Bogoeva, Vanya; Johnsson, Anders Carl G.; Berg, Kristian; Høgset, Anders & Lindgren, Mikael (2018). Photophysical observations on tetrapyrroles during in vitro and in vivo PDT.
• Gederaas, Odrun Arna (2017). Orthotopic glioma model and bladder cancer models on rats-suitable tools for PDT and PCI.
• Gederaas, Odrun Arna (2017). Photochemical techniques available for BioBrilloun members.
• Gederaas, Odrun Arna; Johnsson, Anders Carl G.; Berg, Kristian; Manandhar, Rojlina; Shresta, Chetana & Skåre, Daniel [Vis alle 9 forfattere av denne artikkelen] (2017). Photochemical internalization in bladder cancer – development of an orthotopic in vivo model.
• Gederaas, Odrun Arna; Sharma, Animesh; Bogoeva, Vanya; Mbarak, Saide; Kanamazina, Yusuf & Sporsheim, Bjørnar [Vis alle 9 forfattere av denne artikkelen] (2017). The proteome of endosomes after Bleomycine-mediated photochemical internalization on rat bladder cancer cells.
• Gederaas, Odrun Arna (2017). Photodynamic Therapy – in vitro and in vivo studies using novel photosensitizers and cancer cells.
• Gederaas, Odrun Arna (2017). Photodynamic Therapy (PDT) – novel technologies.
• Gederaas, Odrun Arna (2017). PHOTOBIOLOGY - How to use light in treatment and research .
• Gederaas, Odrun Arna; Johnsson, Anders Carl G.; Berg, Kristian; Manandhar, Rojlina; Shresta, Chetana & Skåre, Daniel [Vis alle 9 forfattere av denne artikkelen] (2016). Photochemical Internalization in bladder cancer-developement of an orthotopic in vivo model.
• Gederaas, Odrun Arna (2016). Photodynamic Therapy (PDT) - Some examples.
• Gederaas, Odrun Arna (2016). Photodynamic Therapy (PDT)-novel technologies.
• Gederaas, Odrun Arna & Qian, Peng (2016). Extracorporeal photopheresis - today and in the future.
• Gederaas, Odrun Arna (2016). Technologies actual for photodyamic therapy and photochemical internalization.
• Gederaas, Odrun Arna (2016). Rapport fra opponent Ass. Professor. Odrun Gederaas, NTNU, vedr. PhD avhandling til Jan Capar, UiT: «Synthesis of Sterically Hindered Macrocyclic Ligands via Demetalation of Metallocorroles» .
• Gederaas, Odrun Arna; Siksjø, Monica; Sæterbø, Kristin G.; Melø, Thor Bernt; Bjørkøy, Astrid & Lindgren, Mikael [Vis alle 7 forfattere av denne artikkelen] (2016). Ruthenium porphyrin-induced photodamage in bladder cancer cells.
• Søgaard, Caroline Danielsen; Petrovic, Voin; Blindheim, Augun; Gederaas, Odrun Arna; Arum, Carl-Jørgen & Otterlei, Marit (2016). PCNA-targeting APIM-peptide alters response to DNA damage in human bladder cancer cells on a signaling level.
• Gederaas, Odrun Arna (2016). Photodynamic Therapy (PDT) by using 5-aminolevululinic (ALA) based photosensitizers.
• Gederaas, Odrun Arna (2016). Research and partnership depending on university structures. Photodynamic Therapy with focus on Photopheresis. .
• Gederaas, Odrun Arna (2016). Enhanced efficacy of Bleomycin in bladder cancer cells by Photochemical Internalization (PCI).
• Gederaas, Odrun Arna; Siksjø, Monica; Mettra, Bastien; Monnereau, Cyrille; Egeberg, Kjartan Wøllo & Andreau, Chanthal [Vis alle 7 forfattere av denne artikkelen] (2015). "Anthracene studies in photodynamic therapy and other ongoing projects". Vis sammendrag

  Anthracene studies in photodynamic therapy and other ongoing projects. Odrun A. Gederaas1, Monica Siksjø2, Bastien Mettra3, Cyrille Monnereau3, Kjartan W. Egeberg1, Chantal Andraud3 and Mikael Lindgren2 1) Department of Cancer Research and Molecular Medicine, University of Science and Technology, N-7489 Trondheim, Norway 2) Department of Physics, N-7491 Trondheim, Norway 3) CNRS UMR 5182, Université Lyon 1, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France Hydrophilic and hydrophobic chromophores are taken up by cells and are usually located in cellular lipid membranes but accumulate in different compartments of the cell. The aim of this study was to investigate biophysical properties of four light sensitive chromophores1,2 for bioimaging applications, and to test their efficiency as potential photosensitizer in photodynamic therapy (PDT). This is a general statement and not verified stringently for all of the probes. The chromophores where all proven to accumulate within tested F98 glioma and CHO-K1 hamster ovary cells. For one of the chromophores (Ant-Poly Imidazole), the polycationic nature of the side chain helped its permeation inside the cell nucleus. Then, characterizations of light induced cell death and DNA damage (Comet assay) were performed using cell survival assay (MTT) and confocal microscopy. Our studies demonstrate structural and morphological changes within the two cell lines upon light/no light treatment after sensitizer or chromophore incubation. Quite surprisingly, one of the hydrophilic anthracene compounds (Ant-Poly Imidazole) although not specifically designed to that end, showed promising results for PDT with accelerated cell death upon illumination compared to the other hydrophilic anthracene compound (Ant-PHEA). Transient absorption measurements revealed a long lived triplet state in one of the tested bromobenzene derivative (DBB2-OAc). The latter also showed generation of singlet oxygen from transient luminescence measurement in the singlet oxygen phosphorescence band, making it a promising candidate for type II PDT. This project is an ongoing collaborating study between ENS Lyon, France, and NTNU, Norway. 1. Monnereau C. et al., New. J. Chem., 2012, 36, 2328-2333. 2. Gallavardin T. et al., Chem. Commun., 2012, 48,1689-169.

• Gederaas, Odrun Arna; Siksjø, Monica; Sæterbø, Kristin Grendstad; Melø, Thor Bernt; Bjørkøy, Astrid & Lindgren, Mikael [Vis alle 7 forfattere av denne artikkelen] (2015). Photochemical reactions after nano particle stimulating. In vitro and in vivo studies. Vis sammendrag

  Photochemical reactions after nano particle stimulating. In vitro and in vivo studies Odrun A. Gederaasa, Monica Siksjøb, Kristin G. Sæterbøb, Thor Bernt Meløb, Astrid Bjørkøyb, Mikael Lindgrenb,c, Vanya Bogoevad a) Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N-7491 Trondheim, Norway b) Department of Physics, Norwegian University of Science and Technology, N-7491 Trondheim, Norway c) Department of Physics, Chemistry and Biology, Linköping University, SE-58183 Linköping, Sweden d) Department Molecular Biology of Cell Cycle Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. “G. Bonchev” Str., Bl. 21, Sofia 1113, Bulgaria Abstract Photodynamic therapy (PDT) is a noninvasive treatment for solid malignant and flat tumors. Both endogen and exogen added photosensitizers1 were used in vitro and in vivo studies according to development of new treatment strategies. The light activated sensitizers catalyze photochemical reactions that produce reactive oxygen species which can cause cancer cell death. The photophysical properties of the photosensitizer ruthenium (II) porphyrin (RuP) have been investigated, along with its PDT efficiency onto rat bladder cancer cells (AY27)2. Optical spectroscopy verified that RuP is capable to activate singlet oxygen via blue and red absorption bands and inter system crossing (ISC) to the triplet state3. In vitro experiments on AY27 indicated increased photo-toxicity of RuP (20 µM, 18 h incubation) after cell illumination (at 435 nm, 13 mW/cm2), as a function of blue light exposure. Cell survival fraction was significantly reduced to 14% after illumination (20 min), RuP (20 µM), whereas the “dark toxicity” of RuP (20 µM) was 83%. Structural and morphological changes of cells were observed, due to RuP accumulation, as well as light-dependent cell death, was registered by confocal microscopy. Flow cytometry verified that PDT-RuP (50 µM) triggered significant photo-induced cellular destruction with photoxicity of (93% ±0.9%). Interestingly, the present investigation of RuP-PDT showed that the dominating mode of cell death is necrosis. RuP “dark toxicity” compared to the conventional chemotherapy drug cisplatin, showed higher toxicity, both evaluated by MTT assay (24 h). In conclusion, the present investigation shows that RuP induces cell damage of bladder cancer cells. 1. Gederaas O. A. et al., Photochem. Photobiol. Sci. 2015; 14(7); 1357-6 2. Bogoeva V. et al., J Photochem Photobiol B. 2015(20); 153:276-280 3. Glimsdal E. et al., J Phys. Chem A. 2009(113); 3311-3320

• Gederaas, Odrun Arna (2015). "Bruk av lys i kreftbehandling og forskning".
• Mathisen, Georg & Gederaas, Odrun Arna (2015). "Lyser opp og opplyser". Seminar arrangert av Odrun Gederaas, IKM (NTNU) med finansiering fra Forskningsrådet. [Avis]. Vedr. Seminar på Rockheim, Tr.heim. 24.9.2015. Internett.
• Gederaas, Odrun Arna (2015). Hva har lyset betydd for mennesket ?
• Bogoeva, Vanya; Lindgren, Mikael; Siksjø, Monica; Sæterbø, Kristin Grendstad; Melø, Thor Bernt & Bjørkøy, Astrid [Vis alle 7 forfattere av denne artikkelen] (2015). Photodynamic therapy by using Ruthenium porphyrin on the AY27 rat bladder cancer cell line. Vis sammendrag

  Abstract. Photodynamic therapy is a noninvasive treatment of solid tumors, using photosensitisers, which after activation by light produce reactive oxygen species that selectively destroy target cells. Current focus highlights the photophysical characteristics and the effects of the treatment of the 2,3,7,8,12,13,17,18-OCTAETHYL-21H, 23H-PORPHINE RUTHENIUM(II) CARBONYL (RuP) upon rat bladder cancer cells (Ay27). Photophysical properties of the porphyrin were assessed using: time resolved spectroscopy, life time measurements. The obtained results have shown that RuP is an excellent photosensitizer, suitable for Photodynamic therapy application (PDT). In vitro experiments indicated that the phototoxic effect of RuP is significantly higher, after irradiation (453nm) with RuP upon Ay27, compared to the cytotoxic effect (due to non-irradiation, without light). Confocal microscopy experiments also confirmed higher apoptotic effect of RuP, after irradiation. Additionally, we found that the effect of RuP is higher than the effect of the conventional chemotherapy drug cisplatin, both evaluated by MTT assay. In conclusion, RuP significantly enhanced its phototoxic effect upon AY27 cells, which indicated a potential for further investigations focused on selective treatment of non-suscle invasive bladder cancer.

• Gederaas, Odrun Arna; Siksjø, Monica; Mettra, Bastien; Monnereau, Cyrille; Egeberg, Kjartan Wøllo & Andraud, Chantal [Vis alle 7 forfattere av denne artikkelen] (2015). Photophysics and in cellulo studies of novel light sensitive chromophores with potential application in Photodynamic therapy. Vis sammendrag

  Photophysics and in cellulo studies of novel light sensitive chromophores with potential application in Photodynamic therapy Odrun A. Gederaas1, Monica Siksjø2, Bastien Mettra3, Cyrille Monnereau3, Kjartan W. Egeberg1, Chantal Andraud3 and Mikael Lindgren2 1) Department of Cancer Research and Molecular Medicine, University of Science and Technology, N-7489 Trondheim, Norway 2) Department of Physics, N-7491 Trondheim, Norway 3) CNRS UMR 5182, Université Lyon 1, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France Hydrophilic and hydrophobic chromophores are taken up by cells and are usually located in cellular lipid membranes but accumulate in different compartments of the cell. The aim of this study was to investigate biophysical properties of four light sensitive chromophores1,2 for bioimaging applications, and to test their efficiency as potential photosensitizer in photodynamic therapy (PDT). This is a general statement and not verified stringently for all of the probes. The chromophores where all proven to accumulate within tested F98 glioma and CHO-K1 hamster ovary cells. For one of the chromophores (Ant-Poly Imidazole), the polycationic nature of the side chain helped its permeation inside the cell nucleus. Then, characterizations of light induced cell death and DNA damage (Comet assay) were performed using cell survival assay (MTT) and confocal microscopy. Our studies demonstrate structural and morphological changes within the two cell lines upon light/no light treatment after sensitizer or chromophore incubation. Quite surprisingly, one of the hydrophilic anthracene compounds (Ant-Poly Imidazole) although not specifically designed to that end, showed promising results for PDT with accelerated cell death upon illumination compared to the other hydrophilic anthracene compound (Ant-PHEA). Transient absorption measurements revealed a long lived triplet state in one of the tested bromobenzene derivative (DBB2-OAc). The latter also showed generation of singlet oxygen from transient luminescence measurement in the singlet oxygen phosphorescence band, making it a promising candidate for type II PDT. This project is an ongoing collaborating study between ENS Lyon, France, and NTNU, Norway. 1. Monnereau C. et al., New. J. Chem., 2012, 36, 2328-2333. 2. Gallavardin T. et al., Chem. Commun., 2012, 48,1689-169.

• Gederaas, Odrun Arna; Hauge, Anette; Ellingsen, Pål Gunnar; Berg, Kristian; Altin, Dag & Bardal, Tora [Vis alle 8 forfattere av denne artikkelen] (2015). Photochemical internalization of bleomycin and temozolomide-in vitro on the glioma cell line F98.
• Gederaas, Odrun Arna (2014). Biostruct kurs, Univ. i Tromsø. "Structur guided drug discovery and design". .
• Gederaas, Odrun Arna (2014). Seminar, Norwegian Graduate School in Structural Biology (Biostruct), Jægtvollen. Planlegging av videre prosjekter med foredragsholdere og arrangører (O. Gederaas har tidligere vert en del av BioStuct sitt styre).
• Gederaas, Odrun Arna; Siksjø, Monica & Lindgren, Mikael (2014). Prosjektmøte med samarbeidspartnere for masterstudent Monica Siksjø: Tittel på oppgave: "Optical microscopy studies of localization of PDT chromophores in glioma cells".
• Gederaas, Odrun Arna (2014). Localization and photodynamic therapy of chromophores targeted for cancer cells.
• Gederaas, Odrun Arna (2014). 1. opponent til PhD Thesis ved Naval Sekkat, University of Geneve, 26. Sept.2014 Tittle: "Optimization of Proteolytically Induced Photosensitizers for Fluorescence Imaging and Photodynamic Therapy".
• Gederaas, Odrun Arna (2014). "In vitro and in vivo models in PDT", related to EU application "Innovative Training Networks (ITN): Call: H2020-MSCA-ITN-2015.
• Gederaas, Odrun Arna (2014). Glioma rat cancer studies using PCI technology.
• Gederaas, Odrun Arna; Berg, Kristian & Hauge, Anette (2014). Glioma rat cancer studies using PCI technology.
• Gederaas, Odrun Arna (2014). "Intracellular studies on rat glioma cancer cell line using novel chromophores". Mandagsmøte, IKM, NTNU.
• Gederaas, Odrun Arna; Høgset, Anders; Berg, Kristian; Lindgren, Mikael & Siksjø, Monica (2014). Intracellular studies on rat glioma cancer cell line using novel chromophores.
• Gederaas, Odrun Arna & Høgset, Anders (2014). Behandling av ulike kreftformer på et tidlig stadium.
• Gederaas, Odrun (2013). Kreftceller lyser opp og dør.
• Siksjø, Monica; Mettra, Bastien; Hauge, Anette; Ellingsen, Pål; Høgset, Anders & Egeberg, Kjartan Wøllo [Vis alle 10 forfattere av denne artikkelen] (2013). Localization and photodynamic therapy of chromophores targeted for cancer cells.
• Hansen, Tom Andre; Lindgren, Mikael & Gederaas, Odrun Arna (2018). Investigation of some compounds and gold nanoparticles for photodynamic therapy. Tom Andre Hansen. Vis sammendrag

  Masteroppgave: Effekter av ulike fotosensitizere på kreftceller. Spekteropptak med forskjellige metoder.

• Moe, Nogaye; Hagen, Lars & Gederaas, Odrun Arna (2018). Bleomycin in endosomes after photochemical internalization (PCI) in bladder cancer cells (AY27). Nogaye Moe.
• Singh, Rinku; Grill, Valdemar Erik Robert; Hals, Ingrid Kathrin & Gederaas, Odrun Arna (2018). On the susceptibility and adaptability of beta cells to stressful conditions: effects of nutrient scarcity, hyperoxia and internalization of amylin. Skipnes Kommunikasjon AS. ISSN 978-82-326-2853-7.
• Orjahan, Sayed; Gederaas, Odrun Arna & Hagen, Lars (2017). Endosomal uptake of Doxorubicin in cancer cells after photochemical internalization. Orjahan Sayed.
• Kanamazina, Yusuf; Gederaas, Odrun Arna & Hagen, Lars (2016). Proteomic analysis of endosomes after photochemical internalization - establishment of new protocol for rat bladder cancer cell line (AY27). Tapir Akademisk Forlag.
• Saide, Mbarak; Gederaas, Odrun Arna & Hagen, Lars (2016). Photochemical internalization by cancer cell lines from rat glioma(F98) and bladder (AY27) and its proteome analyses. Tapir Akademisk Forlag.
• Helander, Linda; Krokan, Hans Einar; Gederaas, Odrun Arna; Johnsson, Anders Carl G. & Chen, Duan (2015). Hexylaminolevulinate Photodynamic Treatment of Cancer Cell Lines. PhD Thesis. Tapir Akademisk Forlag.
• Hauge, Anette; Lindgren, Mikael & Gederaas, Odrun (2013). Amphinex-(PPCS2a)-based photodynamic therapy and photochemical internalization of bleomycin and temozolomide- in vitro studies on the glioma cell line F98. NTNU.

Se alle arbeider i Cristin